2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) is a high-purity chromogenic substrate widely utilized in biochemical and clinical research. It appears as a white to light green crystalline powder and is highly soluble in water. The compound serves as a sensitive reagent for detecting peroxidase activity, where it undergoes oxidation to form a stable, green-colored radical cation (ABTS•+).
This reagent is favored for its high molar absorptivity and stability, making it an excellent choice for quantitative spectrophotometric assays. Its redox properties allow for precise measurements of antioxidant capacity and enzymatic kinetics. Due to its sensitivity and reliability, it is a standard component in various diagnostic kits and laboratory protocols requiring the monitoring of oxidative processes.
• Enzyme-Linked Immunosorbent Assay (ELISA): ABTS is frequently employed as a chromogenic substrate for horseradish peroxidase (HRP) in ELISA applications. Upon reaction with HRP and hydrogen peroxide, it produces a soluble green end-product that can be measured spectrophotometrically at 405 nm or 415 nm. This makes it particularly suitable for high-throughput screening where a clear, stable color change is required for accurate quantification of target analytes.
• Antioxidant Capacity Assays: The compound is a core reagent in the Trolox Equivalent Antioxidant Capacity (TEAC) assay and other total antioxidant status (TAS) tests. By generating the stable ABTS radical cation through oxidation, researchers can measure the ability of various samples—such as food extracts, plasma, or plant polyphenols—to neutralize the radical. The resulting decolorization is proportional to the antioxidant concentration, providing a robust metric for comparative studies.
• Enzyme Kinetics and Glucose Monitoring: Beyond ELISA, ABTS is utilized to study the kinetics of various oxidoreductases, including laccases and glucose oxidase. In blood glucose monitoring systems, it acts as a redox indicator to signal the presence of glucose via a coupled enzymatic reaction. Its low background interference and rapid reaction rate ensure high sensitivity in detecting low concentrations of substrates in complex biological matrices.
• Environmental and Industrial Analysis: The reagent finds application in environmental monitoring for detecting phenolic compounds and other pollutants that interact with peroxidase-like catalysts. It is also used in the textile and paper industries to assess the efficacy of enzymatic bleaching processes. Its versatility across different pH ranges and temperatures makes it a reliable tool for both fundamental research and industrial quality control.
Product Name: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
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